Florencio V. Arce, Jeah E. Dela Concepcion, Katrina Mari C. Mayol, Gerard Lee L. See
Objective: The study aimed to determine the in vitro inhibitory activity of Abutilon indicum (Linn 1836) root extract on α-amylase and α-glucosidase enzymes for the prevention of diabetes, one of the major causes of mortality in the Philippines. Methods: This study utilized soxhlation with 95% ethyl alcohol for the extraction method. Three different concentrations (20 µg/ml, 40 µg/ml and 80 µg/ml) of Abutilon indicum root extracts were prepared. Root extracts, of varying concentrations, were subjected to inhibitory assay for a-amylase and a-glucosidase and quantified using a UV-VIS Spectrophotometer. Absorbance reading was measured at 540 nm and 405 nm for the a-amylase and the a-glucosidase inhibitory assay, respectively. Results: There was a dose-dependent percent inhibition by the extract against α-amylase (8.84%- 26.51%) and α-glucosidase (8.12%- 24.36%). Logarithmic regression analysis revealed the median inhibitory concentration (IC50) of a- amylase (191.64 µg/ml) and a-glucosidase (207.13 µg/ml) with a potency and preference for α-amylase over α-glucosidase inhibition by the A. indicum root extract. Conclusion: The findings suggest that Abutilon indicum root extracts are inhibitors of a-amylase and a-glucosidase enzymes which possibly help reduce postprandial glucose levels. © 2016, International Journal of Toxicological and Pharmacological Research. All rights reserved.
Department of Pharmacy, School of Health Care Professions, University of San Carlos, Nasipit Talamban, Cebu City, 6000, Philippines
