Dexter Manalili, Massimiliano Berardi, Hilde Aardema, Konstantina Asimaki, Raymund Sarmiento, B. Imran Akca
The parallel-plate compression test is one of the simplest ways to measure the mechanical properties of a material. In this test, the Young's modulus (E) and the Poisson's ratio (ν) of the material are determined directly without applying any additional modelling and parameter fitting in the post-processing. This is, however, limited when dealing soft biological materials due to their inherent properties such as being inhomogeneous, microscopic, and overly compliant. By combining an interferometry-assisted parallel-plate compression system and a confocal microscope, we were able to overcome these limitations and measure the E (315 ± 52 Pa) and ν (0.210 ± 0.043) of fixated and permeabilized bovine oocytes. © 2022 OSA - The Optical Society. All rights reserved.
LaserLab, Vrije Universiteit Amsterdam, De Boelelaan, Amsterdam, 1081 HV, Netherlands; Physics Department, University of San Carlos, Talamban, Cebu City, 6000, Philippines; Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 7, Utrecht, 3584 CL, Netherlands; Department of Biology and Environmental Science, University of the Philippines Cebu, Cebu City, 6000, Philippines
