Ju Sung Lee, Leo Adrianne Paje, Joyce P. Rodriguez, Ki Sung Kang, Dae-Hyun Hahm, Jae Suk Shim, Yu-Jin Choi, Sanghyun Lee
Cirsium japonicum var. maackii (CM) has been used to treat certain medical conditions such as hemorrhage, hep-atitis, and hypertension. Cirsimaritin was previously found as the major flavonoid in CM and is said to contribute to its pharmacological effects. There are currently no reports detailing the qualitative and quantitative detection of phytochemical indicators in the aerial parts of CM. Therefore, we developed a method to rapidly identify and quantify cirsimaritin in CM using HPLC/UV, and we optimized and validated this analytical method. The results showed good linearity in the concentration range tested (0.25-0.015 mg/mL, r2 ≥ 0.9999), accuracy (93.9-111.3%), and precision (RSD ≤ 0.59%). The developed method can therefore be used for the rapid evaluation of cirsimaritin in CM. © 2020, Korean Society of Pharmacognosy. All rights reserved.
Department of Plant Science and Technology, Chung-Ang University, Anseong, 17546, South Korea; Department of Biology, University of San Carlos, Cebu City, 6000, Philippines; College of Korean Medicine, Gachon University, Seongnam, 13120, South Korea; Department of Physiology, College of Medicine, Kyung Hee University, Seoul, 02447, South Korea; Imsil Herbal Medicine Association, Imsil, 55955, South Korea; Imsil Cheese & Food Research Institute, Imsil, 55918, South Korea
